| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Jack, Tia M (13)
|
ADCPP |
2:40.22 |
2:39.78 |
-0.44 |
| 2 |
Gilbert, Kendall G (13)
|
ADGLEN |
2:40.45 |
2:40.54 |
0.09 |
| 3 |
Slater, Allison I (13)
|
NESNAP |
2:43.41 |
2:41.58 |
-1.83 |
| 4 |
Ferrie, Jillian E (13)
|
ADSCHE |
2:43.66 |
2:42.15 |
-1.51 |
| 5 |
O'Donnell, Maura E (13)
|
ADEELS |
|
2:46.36 |
- |
| 6 |
Ramgulam, Varshana (13)
|
ADCPP |
2:48.18 |
2:47.59 |
-0.59 |
| 7 |
Tennyson, Valerie L (13)
|
ADGLFY |
2:46.94 |
2:47.83 |
0.89 |
| 8 |
Goh, Julia K (13)
|
NEPVA |
2:45.09 |
2:48.49 |
3.40 |
| 9 |
Boyle, Julia C (13)
|
ADSCHE |
3:00.31 |
2:48.73 |
-11.58 |
| 10 |
Mienaltowski, Allison E (13)
|
ADEELS |
2:50.31 |
2:49.44 |
-0.87 |
| 11 |
Gayle, Isabella C (13)
|
ADCPP |
3:05.94 |
2:49.82 |
-16.12 |
| 12 |
Sands, Samantha B (13)
|
ADCPP |
3:03.47 |
2:53.60 |
-9.87 |
| 13 |
Psilopoulos, Andie J (13)
|
ADHAWK |
2:47.17 |
2:54.30 |
7.13 |
| 14 |
Schloth, Katherine I (13)
|
ADCPP |
2:57.07 |
2:56.66 |
-0.41 |
| 15 |
O'Brien, Raegan M (13)
|
ADUSAM |
3:00.65 |
2:59.52 |
-1.13 |
| 16 |
Thompson, Cora A (13)
|
ADBFIN |
3:04.54 |
3:05.07 |
0.53 |
| 17 |
Napier, Clio A (13)
|
ADBFIN |
3:09.74 |
3:07.61 |
-2.13 |
| 18 |
Michels, Delaney R (13)
|
ADCPP |
3:21.46 |
3:08.01 |
-13.45 |
| 19 |
Fraley, Kaiya R (13)
|
ADBFIN |
3:35.53 |
3:08.14 |
-27.39 |
| 20 |
Ricard, Skylar M (13)
|
ADBFIN |
|
3:10.78 |
- |
| 21 |
Pearson, Emily C (13)
|
ADSCHE |
3:23.67 |
3:14.10 |
-9.57 |
| 22 |
Armstrong, Chloe V (13)
|
ADEELS |
3:31.16 |
3:17.89 |
-13.27 |
| 23 |
Thorp, Helen L (13)
|
NESNAP |
|
3:18.16 |
- |
| 24 |
Boenau, Carlye Ana T (13)
|
ADBFIN |
|
3:20.00 |
- |
| 25 |
Lupe, Emma A (13)
|
ADSCHE |
|
3:20.47 |
- |
| 26 |
Panetta, Sophia L (13)
|
ADSCHE |
|
3:34.91 |
- |